В Главный Параметры Из Объектив Объектив О Оптический Микроскоп

① Magnification refers to the ratio of the size of the image seen by the eye to the size of the corresponding specimen. It refers to the ratio of length rather than the ratio of area. Example: Magnification factor of 100 ×, Refers to a length of 1 μ The length of the magnified specimen of m is 100 μ m. If calculated by area, it имеет увеличено на 10000 раз.

② Numerical aperture, also known as aperture ratio, abbreviated as NA or A, is the main parameter of the objective lens and condenser, and is directly proportional to the resolution of the microscope. The numerical aperture of the dry objective is 0.05-0.95, and the numerical aperture of the oil immersed objective (cedar oil) is 1.25.

optical микроскоп

③ Working distance refers to the distance from the front lens of the objective lens to the cover glass of the specimen when the specimen being observed is the clearest. The working distance of the objective lens is related to its focal length. The longer the focal length of the objective lens, the lower the magnification, and the longer its working distance. Example: A 10x objective is labeled with 10/0.25 and 160/0.17, where 10 is the magnification of the objective; 0.25 is the numerical aperture; 160 is the length of the lens tube (in mm); 0.17 is the standard thickness of the cover glass in millimeters. The effective working distance of a 10x objective is 6.5mm, and the effective working distance of a 40x objective is 0.48mm.

Масло зеркало, один из the оптика микроскопы, is used когда the lens is immersed in oil (обычно кедр масло) to наблюдать finer структуры. It is one of the commonly used микроскопы in the laboratory, with slightly higher clarity than обычный оптический микроскопы, and is used to observe chlamydia, bacteria, organelles, etc

The Lens of the oil lens is very small. When light passes through the air between the glass slide and the oil lens, refraction or total reflection occurs due to different media densities, reducing the amount of light entering the lens and blurring the image. If cedar oil (n=1.515) with a преломление индекс similar to to to of the glass (n=1.7d. 52) IS Добавлено Между The Oil Lens and The Glass Slide, The Amount of Light Ining The Lens Increases, The Brightness of The Field of View Increases, and The Image Becomes Bright and Clear